Addgene - Col1a-3'probe Plasmid Data

Plasmid 20731: Col1a-3'probe

Gene/insert name:
Col1a-3'probe
Insert size:
850
Species:
M. musculus (mouse)
Entrez Gene:
Col1a1 (RP23-112C19.9, Col1a-1, Cola-1, Cola1, Mov-13, Mov13)
Vector backbone:
pBluescriptII KS
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
2970
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Beard et al (Genesis. 2006 Jan . 44(1):23-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20731" in your Materials and Methods section.