Addgene - pAdEasy-EGFP-CLASP2 340-1084 Plasmid Data

Plasmid 24524: pAdEasy-EGFP-CLASP2 340-1084

Gene/insert name:
CLASP2 (340-1084)
Alt name:
CLASP2
Insert size:
3300
Species:
H. sapiens (human)
Entrez Gene:
CLASP2 ()
Fusion protein or tag:
EGFP
Terminal:
N terminal on insert
Mutation:
CLASP2 deletion mutant that retains MT lattice binding and MT plus end tracking activities.
Vector backbone:
pAdEasy-1
(Search Vector Database)
Backbone manufacturer:
Stratagene, ATCC
Vector type:
Mammalian Expression, Adenoviral
Backbone size w/o insert (bp):
33414
5' sequencing primer:
CMV-F, EGFP-C List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Person or lab that originally
cloned the gene/insert:
CLASP2 cDNA was a gift from Anna Akhmanova
Sequence: View sequences (4)
Supplemental document:
CLASP2 Reference sequence. (application/pdf)
Principal Investigator:
Torsten Wittmann
Terms and Licenses:
MTA

Comments:

See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for.

EGFP-CLASP2 340-1084 was cloned into pShuttle-CMV between KpnI and NotI then recombined by homologous recombination into pAdEasy-1.

AdEasy is a registered trademark of the Johns Hopkins University.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: GSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. Kumar et al (J Cell Biol. 2009 Mar 23. 184(6):895-908. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24524" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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