- Backbone size w/o insert (bp) 4501
Vector typeMouse Targeting, Cre/Lox
Growth in Bacteria
Growth instructionsStbl2 (Invitrogen) or other RecA- strain 37 degrees in LB media
Copy numberLow Copy
SpeciesM. musculus (mouse)
Insert Size (bp)3550
Entrez GeneOfd1 (a.k.a. ORF2, Cxorf5, DXGgc7e)
/ Fusion Protein
- Myc (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site ApaI (destroyed during cloning)
- 5′ sequencing primer tcggtgcacatgctttacat (Common Sequencing Primers)
Terms and Licenses
Also see the following article:
Ofd1, a human disease gene, regulates the length and distal structure of centrioles.
Singla V, Romaguera-Ros M, Garcia-Verdugo JM, Reiter JF.
Dev Cell. 2010 Mar 16;18(3):410-24.PMID: 20230748
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFloxin-IRES-Ofd1-Myc was a gift from Jeremy Reiter (Addgene plasmid # 24559)
For your References section:Floxin, a resource for genetically engineering mouse ESCs. Singla V, Hunkapiller J, Santos N, Seol AD, Norman AR, Wakenight P, Skarnes WC, Reiter JF. Nat Methods. 2010 Jan . 7(1):50-2. 10.1038/nmeth.1406 PubMed 19966808
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.