Addgene - pFloxin-IRES-Ofd1-Myc Plasmid Data

Plasmid 24559: pFloxin-IRES-Ofd1-Myc

Gene/insert name:
Ofd1
Insert size:
3550
Species:
M. musculus (mouse)
GenBank ID:
NM_177429
Entrez Gene:
Ofd1 (ORF2, Cxorf5, DXGgc7e)
Fusion protein or tag:
Myc
Terminal:
C terminal on insert
Vector backbone:
pBluescript
(Search Vector Database)
Vector type:
Mouse Targeting, Cre/Lox
Backbone size w/o insert (bp):
4501
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
ApaI
Site destroyed during cloning:
Yes
5' sequencing primer:
tcggtgcacatgctttacat List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl2
Growth temperature (℃):
37
Growth instructions:
Stbl2 (Invitrogen) or other RecA- strain 37 degrees in LB media
High or low copy:
Low Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Jeremy Reiter
Terms and Licenses:
MTA

Comments:

Also see the following article:
Ofd1, a human disease gene, regulates the length and distal structure of centrioles.

Singla V, Romaguera-Ros M, Garcia-Verdugo JM, Reiter JF.

Dev Cell. 2010 Mar 16;18(3):410-24.PMID: 20230748

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Floxin, a resource for genetically engineering mouse ESCs. Singla et al (Nat Methods. 2010 Jan . 7(1):50-2. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24559" in your Materials and Methods section.