|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24586||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typecloning vector
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2733
MutationS167A, S169A, S172A, S173A, S174A, S213A, S217A, S221A
Entrez GeneNFATC4 (a.k.a. NF-AT3, NF-ATC4, NFAT3)
/ Fusion Protein
- EE tag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS_hNFATc4nuc was a gift from Jerry Crabtree (Addgene plasmid # 24586)
For your References section:L-type calcium channels and GSK-3 regulate the activity of NF-ATc4 in hippocampal neurons. Graef IA, Mermelstein PG, Stankunas K, Neilson JR, Deisseroth K, Tsien RW, Crabtree GR. Nature. 1999 Oct 14. 401(6754):703-8. 10.1038/44378 PubMed 10537109
Generated by Addgene from full sequence supplied by depositor.