Addgene - pBS_hNFATc4nuc Plasmid Data

Plasmid 24586: pBS_hNFATc4nuc

Gene/insert name:
NFATc4
Insert size:
2733
Species:
H. sapiens (human)
Entrez Gene:
NFATC4 (NF-ATc4, NFAT3)
Fusion protein or tag:
EE tag
Terminal:
C terminal on insert
Mutation:
S167A, S169A, S172A, S173A, S174A, S213A, S217A, S221A
Vector backbone:
pBS.KS+
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
cloning vector
Backbone size w/o insert (bp):
3000
Cloning site 5':
BamH1
Site destroyed during cloning:
No
Cloning site 3':
Not1
Site destroyed during cloning:
No
5' sequencing primer:
T3 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Principal Investigator:
Jerry Crabtree
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: L-type calcium channels and GSK-3 regulate the activity of NF-ATc4 in hippocampal neurons. Graef et al (Nature. 1999 Oct 14. 401(6754):703-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24586" in your Materials and Methods section.