Addgene - pcDNA4-PKCZeta WT His tagged Plasmid Data

Plasmid 24609: pcDNA4-PKCZeta WT His tagged

Gene/insert name:
PKCZeta
Alt name:
PKC zeta
Species:
H. sapiens (human)
Entrez Gene:
PRKCZ (RP11-181G12.1, PKC-ZETA, PKC2)
Fusion protein or tag:
His
Terminal:
C terminal on insert
Vector backbone:
pcDNA4/TO
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5077
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Zeocin
Sequence: View sequences (2)
Principal Investigator:
Jeff Wrana
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulation of cell polarity and protrusion formation by targeting RhoA for degradation. Wang et al (Science. 2003 Dec 5. 302(5651):1775-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24609" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with