Addgene - pHPyV7-713a Plasmid Data

Plasmid 24728: pHPyV7-713a

Gene/insert name:
Full genome of Human polyomavirus 7
Alt name:
Polyomavirus HPyV7 isolate 713a
Insert size:
4957
Species:
polyomavirus
GenBank ID:
HM011566
Vector backbone:
pFunnyfarm
(Search Vector Database)
Backbone manufacturer:
Christopher Buck lab, Addgene plasmid 24755
Vector type:
Viral clone
Backbone size w/o insert (bp):
2131
Cloning method:
Gateway Cloning
Cloning site 5':
attPI
Site destroyed during cloning:
Yes
Cloning site 3':
AttP2
Site destroyed during cloning:
Yes
5' sequencing primer:
M13/pUC Forward List of Sequencing Primers
3' sequencing primer:
AsyR (CTGAAAGAGGAACTTGGTTAGG)
Bacterial resistance(s)
Bleocin (Zeocin)
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Annotated nucleotide map
Principal Investigator:
Christopher Buck
Terms and Licenses:
MTA

Comments:

genome can be liberated by digestion with Mfe I.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Merkel cell polyomavirus and two previously unknown polyomaviruses are chronically shed from human skin. Schowalter et al (Cell Host Microbe. 2010 Jun 25;7(6):509-15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24728" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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