Addgene - pMCV-R17a Plasmid Data

Plasmid 24729: pMCV-R17a

Gene/insert name:
Full genome of Human Merkel Cell polyomavirus
Insert size:
5391
Species:
polyomavirus
GenBank ID:
HM011566
Vector backbone:
pZErO-2
(Search Vector Database)
Vector type:
Cloning vector
Backbone size w/o insert (bp):
3292
Cloning site 5':
Eco RI
Site destroyed during cloning:
No
Cloning site 3':
Eco RI
Site destroyed during cloning:
No
5' sequencing primer:
M13 reverse primer List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
ccdB Survival
Growth temperature (℃):
37
Growth instructions:
CCDB Survival
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Annotated nucleotide map
Principal Investigator:
Christopher Buck
Terms and Licenses:
MTA

Comments:

genome can be liberated by digestion with EcoRI

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Merkel cell polyomavirus and two previously unknown polyomaviruses are chronically shed from human skin. Schowalter et al (Cell Host Microbe. 2010 Jun 25;7(6):509-15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24729" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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