Addgene - LZRS-DNMT1 Plasmid Data

Plasmid 24952: LZRS-DNMT1

Gene/insert name:
DNMT1
Species:
H. sapiens (human)
Entrez Gene:
DNMT1 (AIM, CXXC9, DNMT, HSN1E, MCMT)
Vector backbone:
LZRS
(Search Vector Database)
Vector type:
Retroviral
Backbone size w/o insert (bp):
11100
Cloning site 5':
EcoRI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
TGGATACACGCCGCCCACGTG List of Sequencing Primers
3' sequencing primer:
ATCGTCGACCACTGTGCTGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
full length WT DNMT1 cDNA from Open Biosystems
Sequence: View sequences (2)
Principal Investigator:
Paul Khavari
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: DNMT1 maintains progenitor function in self-renewing somatic tissue. Sen et al (Nature. 2010 Jan 28. 463(7280):563-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24952" in your Materials and Methods section.