Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24994||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6100
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFgf10 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)6500
Entrez GeneFgf10 (a.k.a. AEY17, BB213776, Fgf-10, Gsfaey17)
/ Fusion Protein
- luciferase (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FGF10-luciferase was a gift from Benoit Bruneau (Addgene plasmid # 24994 ; http://n2t.net/addgene:24994 ; RRID:Addgene_24994)
For your References section:Tbx5 is essential for forelimb bud initiation following patterning of the limb field in the mouse embryo. Agarwal P, Wylie JN, Galceran J, Arkhitko O, Li C, Deng C, Grosschedl R, Bruneau BG. Development. 2003 Feb . 130(3):623-33. 10.1242/dev.00191 PubMed 12490567