Addgene - pT7-EGFP-C1-HsDCP2 Plasmid Data

Plasmid 25031: pT7-EGFP-C1-HsDCP2

Gene/insert name:
DCP2
Insert size:
1263
Species:
H. sapiens (human)
GenBank ID:
AY146650
Entrez Gene:
DCP2 (NUDT20)
Fusion protein or tag:
EGFP
Terminal:
N terminal on backbone
Vector backbone:
pEGFP-C1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4738
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
EGFP-C List of Sequencing Primers
3' sequencing primer:
SV40pA-R
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
Dh5 alpha or XL1-blue
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Elisa Izaurralde
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: DCP1 forms asymmetric trimers to assemble into active mRNA decapping complexes in metazoa. Tritschler et al (Proc Natl Acad Sci U S A. 2009 Dec 22. 106(51):21591-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25031" in your Materials and Methods section.