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pT7-EGFP-C1-HsEDC3
(Plasmid #25032)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25032 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4769
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Dh5 alpha or XL1-blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EDC3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1527
  • GenBank ID
    NM_025083
  • Entrez Gene
    EDC3 (a.k.a. LSM16, MRT50, YJDC, YJEFN2, hYjeF_N2-15q23)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI/BglII (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-EGFP-C1-HsEDC3 was a gift from Elisa Izaurralde (Addgene plasmid # 25032 ; http://n2t.net/addgene:25032 ; RRID:Addgene_25032)
  • For your References section:

    DCP1 forms asymmetric trimers to assemble into active mRNA decapping complexes in metazoa. Tritschler F, Braun JE, Motz C, Igreja C, Haas G, Truffault V, Izaurralde E, Weichenrieder O. Proc Natl Acad Sci U S A. 2009 Dec 22. 106(51):21591-6. 10.1073/pnas.0909871106 PubMed 19966221