PLXNB1
(Plasmid
#25352)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25352 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepFBOH-LIC
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Backbone manufacturerSGC
- Backbone size w/o insert (bp) 6770
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Vector typeBaculovirus expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePLXNB1:Y1511-L2135
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Alt namePDB: 3HM6
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Alt nameplexin B1, cytoplasmic domain
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SpeciesH. sapiens (human)
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Insert Size (bp)1875
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GenBank IDX87904
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Entrez GenePLXNB1 (a.k.a. PLEXIN-B1, PLXN5, SEP)
- Promoter polyhedrin
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pFBOH-Fwd
- 3′ sequencing primer pFBOH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PDB: 3HM6. http://www.thesgc.org/structures/3HM6/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PLXNB1 was a gift from Cheryl Arrowsmith (Addgene plasmid # 25352 ; http://n2t.net/addgene:25352 ; RRID:Addgene_25352)