Addgene: Structural Genomics Consortium Plasmids [Addgene]

The Structural Genomics Consortium is a not-for-profit organization that aims to determine the three dimensional structures of proteins of medical relevance, and place them in the public domain without restriction. Many SGC plasmids from the Toronto and Oxford sites have been deposited at Addgene and are available to request.

Browse the SGC plasmid collection by

Plasmid
Principal Investigator: Cheryl Arrowsmith, Opher Gileadi

Bacterial Expression Plasmids (empty backbones/controls):

Plasmid	GenBank	Key Features
pET15-MHL	EF456738	Hexahistidine tag with TEV cleavage, AmpR
p15TV-L	EF456736	Hexahistidine tag with TEV cleavage, AmpR
pET28a-LIC *	EF442785	Hexahistidine tag with Thrombin cleavage, KanR
pET28GST-LIC	EF456739	GST-tag and hexahistidine tag with Thrombin cleavage, KanR
pET28-MHL	EF456735	Hexahistidine tag with TEV cleavage, KanR
pCW-LIC	EF460848	No tag, double ptac promoter, AmpR
pFBOH-LIC	EF456740	Hexahistidine tag with Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector
pNIC-CH	EF199843	Hexahistidine tag, AmpR
pNIC28-Bsa4	EF198106	Hexahistidine tag with TEV cleavage, KanR
pNIC-CTHF	EF199844	Hexahistidine tag, FLAG tag with TEV cleavage, KanR
pNH-TrxT	GU269914	Hexahistidine tag, thioredoxin with TEV cleavage, KanR
pNIC-ZB	GU452710	Hexahistidine tag , Z-basic, TEV cleavage, AmpR
pFB-LIC-Bse	EF199842	Hexahistidine tag with TEV cleavage, AmpR
pFHMSP-LIC-N	N/A	Expression vector for secreted proteins with N-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR
pFHMSP-LIC-C	N/A	Expression vector for secreted proteins with C-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR

*LIC: Ligation-independent cloning

Related Resources

SGC: http://www.thesgc.org/
SGC Toronto: http://www.sgc.utoronto.ca
SGC Oxford: http://www.sgc.ox.ac.uk/
Protein Database (PDB): http://www.pdb.org

Request Plasmids

To obtain a plasmid, Login or Register a new account. Find your plasmid of interest, then click the Add to Cart button. When you are finished adding plasmids to your cart, complete the checkout process online. Click here for Addgene’s pricing and distribution policy.