Structural Genomics Consortium Plasmids
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The Structural Genomics Consortium is a not-for-profit organization that aims to determine the three dimensional structures of proteins of medical relevance, and place them in the public domain without restriction. Many SGC plasmids from the Toronto, Oxford and Stockholm sites have been deposited at Addgene and are available to request.
| ID | Plasmid | GenBank | Key Features |
|---|---|---|---|
| 26092 | pET15-MHL | EF456738 | Hexahistidine tag with TEV cleavage, AmpR |
| 26093 | p15TV-L | EF456736 | Hexahistidine tag with TEV cleavage, AmpR |
| 26094 | pET28a-LIC | EF442785 | Hexahistidine tag with Thrombin cleavage, KanR |
| 26101 | pET28GST-LIC | EF456739 | GST-tag and hexahistidine tag with Thrombin cleavage, KanR |
| 26096 | pET28-MHL | EF456735 | Hexahistidine tag with TEV cleavage, KanR |
| 26098 | pCW-LIC | EF460848 | No tag, double ptac promoter, AmpR |
| 26099 | pFBOH-LIC | EF456740 | Hexahistidine tag with Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector |
| 26117 | pNIC-CH | EF199843 | Hexahistidine tag, AmpR |
| 26103 | pNIC28-Bsa4 | EF198106 | Hexahistidine tag with TEV cleavage, KanR |
| 26105 | pNIC-CTHF | EF199844 | Hexahistidine tag, FLAG tag with TEV cleavage, KanR |
| 26106 | pNH-TrxT | GU269914 | Hexahistidine tag, thioredoxin with TEV cleavage, KanR |
| 26107 | pNIC-ZB | GU452710 | Hexahistidine tag , Z-basic, TEV cleavage, AmpR |
| 26108 | pFB-LIC-Bse | EF199842 | Hexahistidine tag with TEV cleavage, AmpR |
| 26095 | pFHMSP-LIC-N | N/A | Expression vector for secreted proteins with N-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR |
| 26100 | pFHMSP-LIC-C | N/A | Expression vector for secreted proteins with C-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR |
SGC: http://www.thesgc.org/
SGC Toronto: http://www.sgc.utoronto.ca
SGC Oxford: http://www.sgc.ox.ac.uk/
Protein Database (PDB): http://www.pdb.org