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Browse > Opher Gileadi > Savitsky et al > pFB-LIC-Bse
Plasmid 26108: pFB-LIC-Bse

  • None

  • EF199842

  • His6 - TEV

  • N terminal on backbone

  • pFastBac
    (Search Vector Database)

  • InVitrogen

  • Insect Expression

  • 4803

  • Ligation-Independent cloning; cut with BseRI

  • Yes

  • Ligation-Independent cloning; cut with BseRI

  • Yes

  • FBAC-1 List of Sequencing Primers

  • FBAC-2

  • Ampicillin

  • DH5alpha

  • 37

  • Any E. coli cloning host. To generate bacmids, transform DH10Bac according to the manufacturer's procedure (Invitrogen)

  • High Copy

  • Gentamicin

  • View sequences (3)

  • View map

  • Opher Gileadi

  • MTA

Comments: 

Primers for LIC cloning:

Upstream: add TACTTCCAATCCATG to the 5’ end (ATG in-frame with the desired coding
sequence).

Downstream: add TATCCACCTTTACTG to 5’ end of downstream primer; add termination
codon, if necessary.

Detailed cloning method available in the paper (Savitsky et al., J Struct Biol., 2010).

Generate bacmids and baculoviruses using the Bac to Bac method (Invitrogen)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: High-throughput production of human proteins for crystallization: The SGC experience. Savitsky et al (J Struct Biol. 2010 Jun 10. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26108" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only