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pFB-LIC-Bse
(Plasmid #26108)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26108 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFastBac
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 4803
  • Vector type
    Insect Expression ; baculovirus expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Any E. coli cloning host. To generate bacmids, transform DH10Bac according to the manufacturer's procedure (Invitrogen)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • GenBank ID
    EF199842
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • His6 - TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer fBac-1 (5'-TATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer fBac-2 (5'-GGGAGGTTTTTTAAAGCAAGTAAA-3')
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Primers for LIC cloning:

Upstream: add TACTTCCAATCCATG to the 5’ end (ATG in-frame with the desired coding
sequence).

Downstream: add TATCCACCTTTACTG to 5’ end of downstream primer; add termination
codon, if necessary.

Detailed cloning method available in the paper (Savitsky et al., J Struct Biol., 2010).

Generate bacmids and baculoviruses using the Bac to Bac method (Invitrogen)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFB-LIC-Bse was a gift from Opher Gileadi (Addgene plasmid # 26108)
  • For your References section:

    High-throughput production of human proteins for crystallization: The SGC experience. Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O. J Struct Biol. 2010 Jun 10. ():. 10.1016/j.jsb.2010.06.008 PubMed 20541610