Addgene - pFastBac His6 TEV LIC cloning vector (4B) Plasmid Data

Plasmid 30115: pFastBac His6 TEV LIC cloning vector (4B)

Gene/insert name:
None
Fusion protein or tag:
His6-TEV
Terminal:
N terminal on backbone
Vector backbone:
pFastBac
(Search Vector Database)
Vector type:
Insect Expression
Backbone size (bp):
4812
Cloning site 5':
LIC site
Site destroyed during cloning:
Yes
Cloning site 3':
LIC site
Site destroyed during cloning:
Yes
5' sequencing primer:
LICBac F (5'gtggttggctacgtatactccgg) List of Sequencing Primers
3' sequencing primer:
LICBac R (5'caggttcagggggaggtgtg)
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Gentamicin
Sequence: View sequences (2)
Principal Investigator:
Scott Gradia
Terms and Licenses:
MTA

Comments:

This plasmid is a LIC-adapted pFastBac vector. It uses the same PCR primer tags as with most of our other vectors, so one PCR product can be inserted into many different vectors at once.

This vector will add a His6-TEV sequence to the N terminus of your protein.

Add the following tags to your PCR primers:

LicV1 Forward Tag TACTTCCAATCCAATGCA

LicV1 Reverse Tag TTATCCACTTCCAATGTTATTA

Linearize this plasmid with SspI and gel purify the product, then T4-treat with dGTP. For the PCR product, T4-treat with dCTP.

For more information, please see our website:
http://qb3.berkeley.edu/qb3/macrolab/

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 30115" in your Materials and Methods section.