Addgene - pET28GST-LIC Plasmid Data

Plasmid 26101: pET28GST-LIC

Gene/insert name:
None
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Fusion protein or tag:
6XHis
Terminal:
N terminal on backbone
Fusion protein or tag:
Thrombin CLeavage Site
Terminal:
N terminal on backbone
Vector backbone:
pET28GST-LIC
(Search Vector Database)
Backbone manufacturer:
Structural Genomics Consortium
Vector type:
Bacterial Expression
Backbone size (bp):
7998
5' sequencing primer:
T7, pGEX 5' List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Supplemental document:
pET28GST-LIC (application/pdf)
Principal Investigator:
Cheryl Arrowsmith
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

http://www.sgc.utoronto.ca/SGC-WebPages/toronto...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26101" in your Materials and Methods section.