Purpose(Empty Backbone) SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.
Price$65 (USD)FormatShipped as bacteria in an agar stab at ambient temperature
- Backbone size (bp) 7746
Modifications to backboneDerived from pET15b. Used for T7 promoter driven expression of recombinant proteins with the addition of a 21 amino acid N-terminal fusion tag with 6X His followed by a TEV cleavage site. Two stop codons are included at C-terminal cloning site.
Vector typeBacterial Expression
/ Fusion Proteins
- 6xHis (N terminal on backbone)
- TEV cleavage site (N terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Promoter T7
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
GenBank ID: EF456736.1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p15TV-L was a gift from Cheryl Arrowsmith (Addgene plasmid # 26093)
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.