Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

p15TV-L
(Plasmid #26093)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26093 Plasmid sent as bacteria in agar stab 1 $65 Add to Cart
Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    p15TV-L
  • Backbone manufacturer
    SGC
  • Backbone size (bp) 7746
  • Modifications to backbone
    Derived from pET15b. Used for T7 promoter driven expression of recombinant proteins with the addition of a 21 amino acid N-terminal fusion tag with 6X His followed by a TEV cleavage site. Two stop codons are included at C-terminal cloning site.
  • Vector type
    Bacterial Expression
  • Promoter T7-lacO
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • TEV cleavage site (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Depositor Comments

The p15TV-L vector (GenBank ID: EF456736.1) is a derivative of pET15b (Novagen) and is for infusion based cloning. It is used for T7 promoter driven expression of recombinant proteins with the addition of a 21 amino acid N-terminal fusion tag with 6X His followed by a TEV cleavage site. Two stop codons are included at C-terminal cloning site.

Insertion of DNA sequence into the cloning/expression region is preformed using BD-Biosciences Infusion enzyme mediated directional recombination between complementary 15 nucleotide DNA sequences at the ends of the insert (PCR product) and BseRI linearized vector. Insertion of target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose.

http://www.sgc.utoronto.ca/SGC-WebPages/toronto-vectors.php

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p15TV-L was a gift from Cheryl Arrowsmith (Addgene plasmid # 26093)