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p15TV-L
(Plasmid #26093)

  • Purpose
    (Empty Backbone) SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.
  • Depositing Lab
  • Price
    $65 (USD)
    Format
    Shipped as bacteria in an agar stab at ambient temperature
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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pET15-MHL
  • Backbone manufacturer
    SGC
  • Backbone size (bp) 7746
  • Modifications to backbone
    Derived from pET15b. Used for T7 promoter driven expression of recombinant proteins with the addition of a 21 amino acid N-terminal fusion tag with 6X His followed by a TEV cleavage site. Two stop codons are included at C-terminal cloning site.
  • Vector type
    Bacterial Expression
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • TEV cleavage site (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    None
  • Promoter T7

Cloning Information

Resource Information

Depositor Comments

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p15TV-L was a gift from Cheryl Arrowsmith (Addgene plasmid # 26093)