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(Plasmid #26095)

  • Purpose
    (Empty Backbone) Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
  • Depositing Lab
  • Price
    $65 (USD)
    Shipped as bacteria in an agar stab at ambient temperature
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  • Vector backbone
  • Backbone manufacturer
    Structural Genomics Consortium
  • Vector type
    Insect Expression ; Baculovirus expression
  • Promoter polyhedrin
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • TEV cleavage site (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFHMSP-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFHMSP-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The pFHMSP-LIC N vector is a derivative of the pFastBac HT A vector (Invitrogen). Honeybee melittin signal sequence was introduced before His tag. It is a donor vector for generation of recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector (bacmid) in E. coli host strain, DH10Bac. For use in Bac-to-Bac Baculovirus Expression System in insect cells for secreted protein expression. This vector adds a 26 amino acid N-terminal fusion tag containing 6X His followed by a TEV cleavage site.

Insertion of DNA sequence into the cloning/expression region is performed using BD-Biosciences Infusion enzyme mediated by directional recombination between complementary nucleotide DNA sequences at the ends of the insert (PCR product) and Nco1/HindIII linearized vector. Insertion of target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFHMSP-LIC-N was a gift from Cheryl Arrowsmith (Addgene plasmid # 26095)