Backbone manufacturerStructural Genomics Consortium
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
/ Fusion Protein
- Hexahistidine tag and TEV cleavage, (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
Expression vector for secreted proteins with N-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR
N-terminal tag: AAPEHHHHHHDYDIPTTENLYFQGAMDAA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFHMSP-LIC-N was a gift from Cheryl Arrowsmith (Addgene plasmid # 26095)
Map generated by Addgene from plasmid data.