Addgene - pFHMSP-LIC-N Plasmid Data

Plasmid 26095: pFHMSP-LIC-N

Gene/insert name:
None
Fusion protein or tag:
Hexahistidine tag and TEV cleavage,
Terminal:
N terminal on backbone
Vector backbone:
pFHMSP-LIC-N
(Search Vector Database)
Backbone manufacturer:
Structural Genomics Consortium
Vector type:
Bacterial Expression
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Supplemental document:
pFHMSP-LIC-N/C (application/pdf)
Principal Investigator:
Cheryl Arrowsmith
Terms and Licenses:
MTA

Comments:

Expression vector for secreted proteins with N-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR

N-terminal tag: AAPEHHHHHHDYDIPTTENLYFQGAMDAA

http://www.sgc.utoronto.ca/SGC-WebPages/toronto...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26095" in your Materials and Methods section.