Addgene - pNIC28-Bsa4 Plasmid Data

Plasmid 26103: pNIC28-Bsa4

Gene/insert name:
None
GenBank ID:
EF198106
Fusion protein or tag:
His - TEV
Terminal:
N terminal on backbone
Vector backbone:
pET28a
(Search Vector Database)
Backbone manufacturer:
Novagen
Vector type:
Bacterial Expression
Backbone size (bp):
5353
Cloning site 5':
Ligation-Independent cloning
Site destroyed during cloning:
Yes
Cloning site 3':
Ligation-Independent cloning
Site destroyed during cloning:
Yes
5' sequencing primer:
T7F List of Sequencing Primers
3' sequencing primer:
T7R
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Opher Gileadi
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

Primers for LIC cloning:

Upstream: add TACTTCCAATCCATG to the 5’ end (ATG in-frame with the desired coding
sequence).

Downstream: add TATCCACCTTTACTG to 5’ end of downstream primer; add termination
codon, if necessary.

Detailed cloning method available in the paper (Savitsky et al., J Struct Biol., 2010)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: High-throughput production of human proteins for crystallization: The SGC experience. Savitsky et al (J Struct Biol. 2010 Jun 10. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26103" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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