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Plasmid 26107: pNIC-ZB
  • SGC Empty backbone for bacterial expression

  • None

  • GU452710

  • His6 - Z-basic - TEV

  • N terminal on backbone

  • pET-28-a
    (Search Vector Database)

  • Novagen

  • Bacterial Expression

  • 5515

  • Ligation-Independent cloning; cut with BsaI

  • Yes

  • Ligation-Independent cloning; cut with BsaI

  • Yes

  • T7F List of Sequencing Primers

  • T7R

  • Kanamycin

  • DH5alpha

  • 37

  • For plasmid propagation and cloning, use any E. coli strain that does not express T7 RNA polymerase. For expression, use host cells that express T7 RNA pol, e.g. BL21(DE3).

  • High Copy

  • View sequences (4)
  • Opher Gileadi

  • UBMTA
    EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments: 

Primers for LIC cloning:

Upstream: add TACTTCCAATCCATG to the 5’ end (ATG in-frame with the desired coding
sequence).

Downstream: add TATCCACCTTTACTG to 5’ end of downstream primer; add termination
codon, if necessary.

Detailed cloning method available in the paper (Savitsky et al., J Struct Biol., 2010)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: High-throughput production of human proteins for crystallization: The SGC experience. Savitsky et al (J Struct Biol. 2010 Jun 10. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26107" in your Materials and Methods section.

Price: US $65

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