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pEGFP-mSmo
(Plasmid #25395)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25395 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse Smoothened cDNA
  • Alt name
    Smo
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2382
  • GenBank ID
    NM_176996
  • Entrez Gene
    Smo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HinD III (unknown if destroyed)
  • 3′ cloning site Sal I (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-mSmo was a gift from Philip Beachy (Addgene plasmid # 25395 ; http://n2t.net/addgene:25395 ; RRID:Addgene_25395)
  • For your References section:

    Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened. Chen JK, Taipale J, Cooper MK, Beachy PA. Genes Dev. 2002 Nov 1. 16(21):2743-8. 10.1101/gad.1025302 PubMed 12414725