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pCEFLmGFP-Gli2
(Plasmid #37672)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37672 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCEFL
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gli2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Gli2
  • Promoter EF1a
  • Tag / Fusion Protein
    • mGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer EF1a-Fwd
  • 3′ sequencing primer BGH-Rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

monomeric GFP was constructed by introducing the monomeric mutation, A206K, to EGFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEFLmGFP-Gli2 was a gift from Philip Beachy (Addgene plasmid # 37672 ; http://n2t.net/addgene:37672 ; RRID:Addgene_37672)
  • For your References section:

    Gli2 trafficking links Hedgehog-dependent activation of Smoothened in the primary cilium to transcriptional activation in the nucleus. Kim J, Kato M, Beachy PA. Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21666-71. Epub 2009 Dec 8. 10.1073/pnas.0912180106 PubMed 19996169