|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37672||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
SpeciesM. musculus (mouse)
- Promoter EF1a
/ Fusion Protein
- mGFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer EF1a-Fwd
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypCEFL was provided by J. Silvio Gutkind (National Institutes of Health).
Terms and Licenses
Articles Citing this Plasmid
monomeric GFP was constructed by introducing the monomeric mutation, A206K, to EGFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCEFLmGFP-Gli2 was a gift from Philip Beachy (Addgene plasmid # 37672 ; http://n2t.net/addgene:37672 ; RRID:Addgene_37672)
For your References section:Gli2 trafficking links Hedgehog-dependent activation of Smoothened in the primary cilium to transcriptional activation in the nucleus. Kim J, Kato M, Beachy PA. Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21666-71. Epub 2009 Dec 8. 10.1073/pnas.0912180106 PubMed 19996169