Addgene - pSSec7-mGFPx3 Plasmid Data

Plasmid 25449: pSSec7-mGFPx3

Gene/insert name:
Sec7
Insert size:
6000
Species:
S. cerevisiae (budding yeast)
Entrez Gene:
SEC7 ()
Fusion protein or tag:
mGFPx3
Terminal:
C terminal on insert
Mutation:
GFP contains A206K for monomerization.
Vector backbone:
N/A
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
7700
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
URA3
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Benjamin Glick
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Golgi maturation visualized in living yeast. Losev et al (Nature. 2006 Jun 22. 441(7096):1002-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25449" in your Materials and Methods section.