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pSSec7-mGFPx3
(Plasmid #25449)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 7700
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    Sec7
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    6000
  • Mutation
    GFP contains A206K for monomerization.
  • Entrez Gene
    SEC7
  • Tag / Fusion Protein
    • mGFPx3 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSSec7-mGFPx3 was a gift from Benjamin Glick (Addgene plasmid # 25449)
  • For your References section:

    Golgi maturation visualized in living yeast. Losev E, Reinke CA, Jellen J, Strongin DE, Bevis BJ, Glick BS. Nature. 2006 Jun 22. 441(7096):1002-6. 10.1038/nature04717 PubMed 16699524