1
Plasmid 25449: pSSec7-mGFPx3
  • Sec7

  • 6000

  • S. cerevisiae (budding yeast)

  • SEC7 ()

  • mGFPx3

  • C terminal on insert

  • GFP contains A206K for monomerization.

  • N/A
    (Search Vector Database)

  • Yeast Expression

  • 7700

  • EcoRI

  • No

  • BamHI

  • No

  • n/a List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • URA3

  • View sequences (4)
  • View map

  • Benjamin Glick

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Golgi maturation visualized in living yeast. Losev et al (Nature. 2006 Jun 22. 441(7096):1002-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25449" in your Materials and Methods section.