Addgene - pBAD-LSSmOrange Plasmid Data

Plasmid 37129: pBAD-LSSmOrange

Gene/insert name:
LSSmOrange
Insert size:
710
Fusion protein or tag:
His
Terminal:
N terminal on backbone
Mutation:
A44V/F84L/W145M/I165D/M167L/G202D compared to mOrange (but numbering relative to EGFP)
Vector backbone:
pBAD/His-B
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3999
Modifications to Backbone:
Several amino acids between His-tag and LSSmOrange were removed.
Promoter:
araB
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pBAD-fwd List of Sequencing Primers
3' sequencing primer:
pBAD-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Vladislav Verkhusha
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An Orange Fluorescent Protein with a Large Stokes Shift for Single-Excitation Multicolor FCCS and FRET Imaging. Shcherbakova et al (J Am Chem Soc. 2012 Apr 24. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37129" in your Materials and Methods section.