|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31909||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3999
Modifications to backboneSeveral amino acids between His-tag and LSSmKate2 were removed.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)708
MutationK69Y/P131T/S148G/M167D/T183S/M196V compared to mKate but numbering is relative to EGFP.
/ Fusion Protein
- HIS (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pBAD-fwd
- 3′ sequencing primer pBAD-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBAD/HisD-LSSmKate2 was a gift from Vladislav Verkhusha (Addgene plasmid # 31909 ; http://n2t.net/addgene:31909 ; RRID:Addgene_31909)
For your References section:Monomeric red fluorescent proteins with a large Stokes shift. Piatkevich KD, Hulit J, Subach OM, Wu B, Abdulla A, Segall JE, Verkhusha VV. Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5369-74. Epub 2010 Mar 8. 10.1073/pnas.0914365107 PubMed 20212155