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mCardinal-pBAD
(Plasmid #54800)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 54800 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    mCardinal-pBAD
  • Backbone size (bp) 4800
  • Vector type
    Bacterial Expression
  • Tags / Fusion Proteins
    • mCardinal (N terminal on backbone)
    • 6xHis (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • mCardinal (N terminal on backbone)
    • 6xHis (N terminal on backbone)

Resource Information

  • Terms and Licenses

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 604; Emission = 659

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCardinal-pBAD was a gift from Michael Davidson (Addgene plasmid # 54800)
  • For your References section:

    Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein. Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia KC, Contag CH, Shen K, Blau HM, Lin MZ. Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888. 10.1038/nmeth.2888 PubMed 24633408