pCS2 mouse CV2 PC
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25776||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecross veinless 2
SpeciesM. musculus (mouse)
Insert Size (bp)2300
Entrez GeneBmper (a.k.a. Cv2, CV-2, Crim3, 3110056H04Rik)
/ Fusion Protein
- PC (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
For sense, linearize with NotI, transcribe with SP6.
Protein C Epitope tag sequence - EDQVDPRLIDGK
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2 mouse CV2 PC was a gift from Edward De Robertis (Addgene plasmid # 25776 ; http://n2t.net/addgene:25776 ; RRID:Addgene_25776)
For your References section:Mouse Crossveinless-2 is the vertebrate homolog of a Drosophila extracellular regulator of BMP signaling. Coffinier C, Ketpura N, Tran U, Geissert D, De Robertis EM. Mech Dev. 2002 Dec . 119 Suppl 1():S179-84. 10.1016/S0925-4773(03)00113-8 PubMed 14516682
Map uploaded by the depositor.