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pCS2 xenopus Insulin 2
(Plasmid #25786)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25786 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Insulin 2
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    340

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Antisense: cut with EcoRI, transcribe with T7

Sense: cut with NotI, transcribe with SP6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 xenopus Insulin 2 was a gift from Edward De Robertis (Addgene plasmid # 25786 ; http://n2t.net/addgene:25786 ; RRID:Addgene_25786)
  • For your References section:

    Isthmin is a novel secreted protein expressed as part of the Fgf-8 synexpression group in the Xenopus midbrain-hindbrain organizer. Pera EM, Kim JI, Martinez SL, Brechner M, Li SY, Wessely O, De Robertis EM. Mech Dev. 2002 Aug . 116(1-2):169-72. 10.1016/S0925-4773(02)00123-5 PubMed 12128218