Addgene - pcDNA3-LAP2-YFP-NLS Plasmid Data

Plasmid 25847: pcDNA3-LAP2-YFP-NLS

Gene/insert name:
LAP2-YFP-NLS
Alt name:
Coumarin PRIME positive control plasmid
Insert size:
867
Fusion protein or tag:
His
Terminal:
N terminal on backbone
Fusion protein or tag:
NLS
Terminal:
C terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Principal Investigator:
Alice Ting
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A fluorophore ligase for site-specific protein labeling inside living cells. Uttamapinant et al (Proc Natl Acad Sci U S A. 2010 Jun 15. 107(24):10914-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25847" in your Materials and Methods section.