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myc-Rapr-FAK
(Plasmid #25926)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    RapR-FAK
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3800
  • GenBank ID
    NM_007982
  • Entrez Gene
    Ptk2 (a.k.a. FAK, FRNK, Fadk, mKIAA4203, p125FAK)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer CMV-Fw
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    myc-Rapr-FAK was a gift from Klaus Hahn (Addgene plasmid # 25926)
  • For your References section:

    Engineered allosteric activation of kinases in living cells. Karginov AV, Ding F, Kota P, Dokholyan NV, Hahn KM. Nat Biotechnol. 2010 Jun 27. ():. 10.1038/nbt.1639 PubMed 20581846