Addgene - myc-Rapr-FAK Plasmid Data

Plasmid 25926: myc-Rapr-FAK

Gene/insert name:
RapR-FAK
Insert size:
3800
Species:
M. musculus (mouse)
GenBank ID:
NM_007982
Entrez Gene:
Ptk2 (FAK, FRNK, Fadk, mKIAA4203, p125FAK)
Fusion protein or tag:
Myc
Terminal:
N terminal on insert
Vector backbone:
pRc/CMV
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
BglII
Site destroyed during cloning:
No
5' sequencing primer:
CMV-Fw List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Klaus Hahn
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Engineered allosteric activation of kinases in living cells. Karginov et al (Nat Biotechnol. 2010 Jun 27. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25926" in your Materials and Methods section.