pUSE-RapR-Src(KD)-myc
(Plasmid #25934)

• Depositing Lab: Klaus Hahn
• Publication: Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Backbone

• Vector backbone: pUSE
• Backbone size w/o insert (bp): 5700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RapR-Src
• Alt name: FKBP1a
• Alt name: FKPB12
• Species: M. musculus (mouse)
• Insert Size (bp): 1900
• Mutation: This pUSE-RapR-Src(KD)-myc plasmid contains the kinase inactive mutation D388R. The uploaded sequence data is based on wt.
• Entrez Gene: Fkbp1a (a.k.a. FKBP12, Fkbp, Fkbp1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-Fw

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry