Addgene - myc-Rictor corrected Plasmid Data

Plasmid 11367: myc-Rictor corrected

Gene/insert name:
Rictor
Insert size:
5700
Species:
H. sapiens (human)
Entrez Gene:
RICTOR (PIA, mAVO3)
Fusion protein or tag:
Myc
Terminal:
N terminal on backbone
Vector backbone:
pRK-5
(Search Vector Database)
Backbone manufacturer:
BD Pharmingen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4754
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3
High or low copy:
High Copy
Sequence: View sequences (3)
Supplemental document:
Notes from Addgene (2)
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Comments:

The amino acid sequence of this plasmid matches the NCBI published sequence. No functional differences between the plasmid with the point mutations (Addgene #1860) and this plasmid have been observed thus far. In this version, silent point mutations in the N-terminus were created to destroy the NotI site and facilitate cloning. These will not change the amino acid sequence of the protein. The ORF sequence of the new insert has been uploaded and is linked from this datasheet.

Addgene has compiled full sequence for this plasmid. Please see Notes link.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Sarbassov et al (Curr Biol 2004 Jul 27;14(14):1296-302. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11367" in your Materials and Methods section.