Addgene - HA Raptor Plasmid Data

Plasmid Cart

Login to add to cart

Recently Visited

8513: HA Raptor
8455: pCMV-dR8.2 d…
8454: pCMV-VSV-G
8453: pLKO.1 puro
8408: p255 pPr-CRE…

Popular Plasmids

Special Collections

Analyze Sequence

Vector Database

Plasmid 8513: HA Raptor

Gene/insert name:
Raptor
Insert size:
6000
Species:
H. sapiens (human)
Entrez Gene:
RPTOR (KOG1, Mip1, KIAA1303)
Fusion protein or tag:
ha
Terminal:
N terminal on backbone
Vector backbone:
prk5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4716
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
SV40pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Comments:

Addgene has compiled full sequence for this plasmid. Please see Notes link.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Kim et al (Cell 2002 Jul 26;110(2):163-75. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8513" in your Materials and Methods section.