Addgene - HA-GbL Plasmid Data

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1865: HA-GbL
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Plasmid 1865: HA-GbL

Gene/insert name:
GbL
Alt name:
G beta L
Alt name:
G protein beta subunit-like
Species:
H. sapiens (human)
Entrez Gene:
MLST8 (GBL, LST8, POP3, WAT1, GbetaL, MGC111011)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pRK-5
(Search Vector Database)
Backbone manufacturer:
BD Pharmingen
Vector type:
Mammalian Expression
Backbone size w/o insert:
4754
Cloning site 5':
SalI
Site destroyed during cloning:
Unknown
Cloning site 3':
NotI
Site destroyed during cloning:
Unknown
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
SV40pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Sarbassov et al (Curr Biol 2004 Jul 27;14(14):1296-302. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1865" in your Materials and Methods section.