PurposePlease see Addgene item #11367 for an updated version of this plasmid.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1860||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBD Pharmingen
- Backbone size w/o insert (bp) 4754
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneRICTOR (a.k.a. AVO3, PIA, hAVO3)
/ Fusion Protein
- Myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site XmaI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Please note: This plasmid contains 2 amino acids that are different than the published NCBI sequence. The changes can be viewed in the Addgene Full Sequence link above- T->C at bp 1312 resulting in amino acid 35 being changed from S->P and a T->C change at bp 1556 resulting in amino acid 116 being changed from I->T. A new version of this plasmid with the corrected sequence is available (Addgene #11367).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:myc-Rictor was a gift from David Sabatini (Addgene plasmid # 1860 ; http://n2t.net/addgene:1860 ; RRID:Addgene_1860)
For your References section:Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Sarbassov DD, Ali SM, Kim DH, Guertin DA, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM. Curr Biol 2004 Jul 27;14(14):1296-302. 10.1016/j.cub.2004.06.054 PubMed 15268862