Addgene - pcDNA3-Flag mTOR wt Plasmid Data

Plasmid 26603: pcDNA3-Flag mTOR wt

Gene/insert name:
mTOR
Insert size:
7650
Species:
H. sapiens (human)
GenBank ID:
NM_004958
Entrez Gene:
MTOR (FRAP, FRAP1, FRAP2, RAFT1, RAPT1)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
WT mTOR insert is from Stuart Schreiber's lab. Brown et al, Nature 369:756 (1994).
Sequence: View sequences (3)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Jie Chen
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The FKBP12-rapamycin-binding domain is required for FKBP12-rapamycin-associated protein kinase activity and G1 progression. Vilella-Bach et al (J Biol Chem. 1999 Feb 12. 274(7):4266-72. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26603" in your Materials and Methods section.