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Plasmid 26603: pcDNA3-Flag mTOR wt
  • Mammalian expression of human flag-tagged mTor

  • mTOR

  • 7650

  • H. sapiens (human)

  • NM_004958

  • MTOR (FRAP, FRAP1, FRAP2, RAFT1, RAPT1)

  • Flag

  • N terminal on insert

  • pcDNA3
    (Search Vector Database)

  • Invitrogen

  • Mammalian Expression

  • 5400

  • NotI

  • No

  • NotI

  • No

  • CMV-F List of Sequencing Primers

  • BGH-rev

  • Ampicillin

  • DH5alpha

  • 37

  • Low Copy

  • Neomycin

  • WT mTOR insert is from Stuart Schreiber's lab. Brown et al, Nature 369:756 (1994).

  • View sequences (3)
  • Notes from Addgene (1)

  • Jie Chen

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The FKBP12-rapamycin-binding domain is required for FKBP12-rapamycin-associated protein kinase activity and G1 progression. Vilella-Bach et al (J Biol Chem. 1999 Feb 12. 274(7):4266-72. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26603" in your Materials and Methods section.