|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26020||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5428
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nametailless vinculinTS
Insert Size (bp)4206
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH (Common Sequencing Primers)
Please note that the sequence provided by the depositor for this plasmid is for VinculinTS. Please refer to Addgene's quality control sequence with EGFP-C primer for the correct plasmid sequence downstream of Venus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Tailless VinculinTS was a gift from Martin Schwartz (Addgene plasmid # 26020 ; http://n2t.net/addgene:26020 ; RRID:Addgene_26020)
For your References section:Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA. Nature. 2010 Jul 8. 466(7303):263-6. 10.1038/nature09198 PubMed 20613844