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pMXs-Ms-c-Myc-W136E
(Plasmid #26024)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26024 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMXs
  • Backbone manufacturer
    Dr. Toshio Kitamura of the University of Tokyo
  • Backbone size w/o insert (bp) 6340
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-Myc
  • Alt name
    Myc2; Nird; Niard; Myc
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1320
  • Mutation
    W136E
  • Entrez Gene
    Myc (a.k.a. AU016757, Myc2, Niard, Nird, bHLHe39)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 (not destroyed)
  • 3′ cloning site attR2 (not destroyed)
  • 5′ sequencing primer pMX-S1811 (GAC GGC ATC GCA GCT TGG ATA CAC)
  • 3′ sequencing primer pMX-TgAS (GAC ATG GCC TGC CCG GTT ATT ATT)
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Mutation(s) made by Provider lab: W136E
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-Ms-c-Myc-W136E was a gift from Shinya Yamanaka (Addgene plasmid # 26024)
  • For your References section:

    Promotion of direct reprogramming by transformation-deficient Myc. Nakagawa M, Takizawa N, Narita M, Ichisaka T, Yamanaka S. Proc Natl Acad Sci U S A. 2010 Jul 26. ():. 10.1073/pnas.1009374107 PubMed 20660764