Addgene - pMXs-Ms-L-Myc Plasmid Data

Plasmid 26023: pMXs-Ms-L-Myc

Gene/insert name:
L-Myc
Alt name:
L-myc; Lmyc1; Lmyc-1; Mycl1
Insert size:
1107
Species:
M. musculus (mouse)
Entrez Gene:
Mycl1 (RP23-121J14.5, AW536278, L-myc, Lmyc-1, Lmyc1, bHLHe38)
Mutation:
None
Vector backbone:
pMXs
(Search Vector Database)
Backbone manufacturer:
Dr. Toshio Kitamura of the University of Tokyo
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6340
Cloning method:
Gateway Cloning
Cloning site 5':
attR1
Site destroyed during cloning:
No
Cloning site 3':
attR2
Site destroyed during cloning:
No
5' sequencing primer:
pMX-S1811 (GAC GGC ATC GCA GCT TGG ATA CAC) List of Sequencing Primers
3' sequencing primer:
pMX-TgAS (GAC ATG GCC TGC CCG GTT ATT ATT)
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Promotion of direct reprogramming by transformation-deficient Myc. Nakagawa et al (Proc Natl Acad Sci U S A. 2010 Jul 26. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26023" in your Materials and Methods section.