476 p5E-mcs
(Plasmid #26029)

Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attL1 (not destroyed)
  • 3′ cloning site attR2 (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

"p5E" refers to 5' entry clones flanked by L4-R1

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    476 p5E-mcs was a gift from Nathan Lawson (Addgene plasmid # 26029)