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599 p3E-mcs1
(Plasmid #49004)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49004 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDONRP2RP3
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 2639
  • Vector type
    gateway 3' Entry

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Multiple Cloning Site
  • Insert Size (bp)
    26

Cloning Information

Resource Information

Depositor Comments

There is a single base mismatch between Addgene's quality control sequence and the full plasmid reference sequence. The change is in backbone region and should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    599 p3E-mcs1 was a gift from Nathan Lawson (Addgene plasmid # 49004)
  • For your References section:

    Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N. Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 10.1016/j.ydbio.2013.08.028. 10.1016/j.ydbio.2013.08.028 PubMed 24036310