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512 pME-miR
(Plasmid #26032)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26032 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ef1a
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    753
  • Entrez Gene
    eef1a1l1 (a.k.a. EFL1-alpha, chunp6927, eef1a, ef1a, ik:tdsubc_2a3, ik:tdsubc_2b3, tdsubc_2a3, wu:fa91c07, wu:fa94b03, wu:fi13b09, xx:tdsubc_2a3, xx:tdsubc_2b3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site attL1 (not destroyed)
  • 3′ cloning site attL2 (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

EF1alpha gene insert (also known as ef1a, accession number NM_131263) encompassing the TATA box, first non-coding exon 1, intron 1 and partial exon 2, 5′ of the ATG.

There are minor mismatches in the ef1a region. These changes should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    512 pME-miR was a gift from Nathan Lawson (Addgene plasmid # 26032)
  • For your References section:

    MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis. Nicoli S, Standley C, Walker P, Hurlstone A, Fogarty KE, Lawson ND. Nature. 2010 Apr 22. 464(7292):1196-200. 10.1038/nature08889 PubMed 20364122