1
Plasmid 26032: 512 pME-miR
  • ef1a

  • 753

  • D. rerio (zebrafish)

  • eef1a1l1 (EFL1-alpha, chunp6927, eef1a, ef1a, ik:tdsubc_2a3, ik:tdsubc_2b3, tdsubc_2a3, wu:fa91c07, wu:fa94b03, wu:fi13b09, xx:tdsubc_2a3, xx:tdsubc_2b3)

  • pME
    (Search Vector Database)

  • Entry vector

  • attL1

  • No

  • attL2

  • No

  • M13pUC-fwd List of Sequencing Primers

  • M13rev

  • Kanamycin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • Nathan Lawson

  • UBMTA

Comments: 

EF1alpha gene insert (also known as ef1a, accession number NM_131263) encompassing the TATA box, first non-coding exon 1, intron 1 and partial exon 2, 5′ of the ATG.

There are minor mismatches in the ef1a region. These changes should not affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis. Nicoli et al (Nature. 2010 Apr 22. 464(7292):1196-200. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26032" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only