Addgene - pJFRC7-20XUAS-IVS-mCD8::GFP Plasmid Data

Plasmid 26220: pJFRC7-20XUAS-IVS-mCD8::GFP

Gene/insert name:
mCD8::GFP
Insert size:
1389
Species:
M. musculus (mouse)
Vector backbone:
pJFRC-MUH
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
8151
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
hsp70 F: GAGCGCCGGAGTATAAATAGAG List of Sequencing Primers
3' sequencing primer:
SV40 R: CCATTCATCAGTTCCATAGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Supplemental document:
Author's PDF Map of pJFRC7-20XUAS-IVS-mCD8::GFP (application/pdf)
Principal Investigator:
Gerald Rubin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Refinement of Tools for Targeted Gene Expression in Drosophila. Pfeiffer et al (Genetics. 2010 Aug 9. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26220" in your Materials and Methods section.