Addgene - pBPGAL4.2Uw-2 Plasmid Data

Plasmid 26227: pBPGAL4.2Uw-2

Gene/insert name:
GAL4
Insert size:
2646
Species:
S. cerevisiae (budding yeast)
Entrez Gene:
GAL4 (YPL248C, GAL81)
Vector backbone:
pBPGUw
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
9223
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
DSCP F: GAGCTCGCCCGGGGATC List of Sequencing Primers
3' sequencing primer:
GAL4 R: CGGCATCCTTGTTGACGTTAT
Bacterial resistance(s)
Ampicillin and Chloramphenicol
Growth strain(s)
ccdB Survival
Growth temperature (℃):
37
Growth instructions:
ccdB Survival 2T1 strain (Invitrogen), or equivalent, must be used to propagate plasmids carrying the ccdB gene.
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Gerald Rubin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Refinement of Tools for Targeted Gene Expression in Drosophila. Pfeiffer et al (Genetics. 2010 Aug 9. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26227" in your Materials and Methods section.