|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26248||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerconstructed in Cluzel Lab
- Backbone size w/o insert (bp) 2600
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namems2 binding sites (x2)
Insert Size (bp)200
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (destroyed during cloning)
- 3′ cloning site HindIII (destroyed during cloning)
- 5′ sequencing primer PLtetO-1 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe tandem ms2 binding sites were cloned from the pIIIMS2-2 plasmid, which was a gift from K Bloom and UNC Chapel Hill. DsRedT3f was cloned from pQE31-DsRed.T3f from Dan Strongnin and the Glick Lab, U Chicago The vector was a gift from H. Bujard to C. Guet.
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pZE31-DsRed-ms2x2 was a gift from Philippe Cluzel (Addgene plasmid # 26248 ; http://n2t.net/addgene:26248 ; RRID:Addgene_26248)
For your References section:Minimally invasive determination of mRNA concentration in single living bacteria. Guet CC, Bruneaux L, Min TL, Siegal-Gaskins D, Figueroa I, Emonet T, Cluzel P. Nucleic Acids Res. 2008 Jul . 36(12):e73. 10.1093/nar/gkn329 PubMed 18515347