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pZS*12-MS2-GFP
(Plasmid #26245)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26245 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pZS*12
  • Backbone manufacturer
    Lutz and Bujard, Nucleic Acids Research 25 (1997) 1203-1210.
  • Backbone size w/o insert (bp) 3659
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MS2-GFP
  • Alt name
    MS2 coat protein and GFP fusion
  • Species
    fusion
  • Insert Size (bp)
    1083
  • Mutation
    five amino acid linker (Ser, Gly, Gly, Gly, Gly) between the C terminus of the dlFG mutant MS2 coat protein and the N terminus of GFP. The MS2 coat protein is a dlFG mutant with a deletion for amino acids 68-82.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer PLlacO-1
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZS*12-MS2-GFP was a gift from Philippe Cluzel (Addgene plasmid # 26245 ; http://n2t.net/addgene:26245 ; RRID:Addgene_26245)
  • For your References section:

    Minimally invasive determination of mRNA concentration in single living bacteria. Guet CC, Bruneaux L, Min TL, Siegal-Gaskins D, Figueroa I, Emonet T, Cluzel P. Nucleic Acids Res. 2008 Jul . 36(12):e73. 10.1093/nar/gkn329 PubMed 18515347