|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27118||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3422
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameMS2 binding sites (6)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer M13_Rev (Common Sequencing Primers)
The Singer Lab no longer recommends this plasmid, as it has a higher rate of bacterial recombination.
For mammalian cells they recommend the following plasmids:
pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre (Plasmid #84561)
HaloTag-bActinCDS-bActinUTR-MS2V5 (Plasmid #102718)
For yeast cells they recommend the following plasmids:
pET246-pUC57 12xMS2V6 (Plasmid #104390)
pET259-pUC57 24xMS2V6 (Plasmid #104391)
pET251-pUC 12xMS2V6 Loxp KANr Loxp (Plasmid #104392)
pET264-pUC 24xMS2V6 Loxp KANr Loxp (Plasmid #104393)
Loop sequence: acatgaggatcacccatgt
Addgene's sequencing result contains 1 nucleotide mismatch at bp# 345 when compared to the full plasmid sequence provided by the depositing laboratory.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSL-MS2-6X was a gift from Robert Singer (Addgene plasmid # 27118 ; http://n2t.net/addgene:27118 ; RRID:Addgene_27118)
For your References section:Localization of ASH1 mRNA particles in living yeast. Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM. Mol Cell. 1998 Oct . 2(4):437-45. 10.1016/S1097-2765(00)80143-4 PubMed 9809065