Addgene - pSL-MS2-12X Plasmid Data

Plasmid 27119: pSL-MS2-12X

Gene/insert name:
MS2 binding sites (12)
Vector backbone:
pSL1180
(Search Vector Database)
Backbone manufacturer:
Pharmacia
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3422
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BglII
Site destroyed during cloning:
No
5' sequencing primer:
M13_Rev List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl2
Growth temperature (℃):
30
Growth instructions:
Stbl2
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Robert Singer
Terms and Licenses:
MTA

Comments:

Loop sequence: acatgaggatcacccatgt

Our sequencing result contains nucleotide mismatches at bp# 345 and #673 when compared to sequence provided by the laboratory.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Localization of ASH1 mRNA particles in living yeast. Bertrand et al (Mol Cell. 1998 Oct . 2(4):437-45. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27119" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

27121: pMS2-GFP

27122: pMS2-YFP

27118: pSL-MS2-6X

31865: pCR4-24XMS2SL-stable

27117: pG14-MS2-GFP