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MS2-HB
(Plasmid #35573)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35573 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pQCXIP
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 7480
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MS2 Coat Protein
  • Alt name
    N55K
  • Species
    Bacteriophage
  • Insert Size (bp)
    777
  • Mutation
    Insert is a tail-to-head tandem placement of two MS2 coat protein open reading frames. Both MS2 contains a N55K mutation that increases protein affinity to MS2 stem loop
  • Entrez Gene
    cp (a.k.a. MS2g2)
  • Promoter CMV
  • Tag / Fusion Protein
    • HTBH (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site Pac1 (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    MS2 coat protein sequence was amplified from pCT119-N55K (gift from David Peabody, University of New Mexico).
  • Terms and Licenses
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MS2-HB was a gift from Marian Waterman (Addgene plasmid # 35573 ; http://n2t.net/addgene:35573 ; RRID:Addgene_35573)
  • For your References section:

    Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel integrated proteomic approach. Tsai BP, Wang X, Huang L, Waterman ML. Mol Cell Proteomics. 2011 Apr;10(4):M110.007385. Epub 2011 Feb 1. 10.1074/mcp.M110.007385 PubMed 21285413