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Tagged-IRES
(Plasmid #35570)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35570 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSTF
  • Backbone manufacturer
    Jang et al. 2004
  • Backbone size w/o insert (bp) 4660
  • Modifications to backbone
    Renilla Luciferase open reading frame removed. 4 Ms2 stem loop sequences cloned downstream of Firefly Luciferase.
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lymphoid Enhancer Factor 1 5'UTR
  • Alt name
    LEF1 IRES
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1183
  • Entrez Gene
    LEF1 (a.k.a. LEF-1, TCF10, TCF1ALPHA, TCF7L3)
  • Promoter SV40
  • Tags / Fusion Proteins
    • Firefly Luciferase (C terminal on insert)
    • MS2 Stem Loop (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Afe1 (destroyed during cloning)
  • 3′ cloning site Afe1 (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tagged-IRES was a gift from Marian Waterman (Addgene plasmid # 35570 ; http://n2t.net/addgene:35570 ; RRID:Addgene_35570)
  • For your References section:

    Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel integrated proteomic approach. Tsai BP, Wang X, Huang L, Waterman ML. Mol Cell Proteomics. 2011 Apr;10(4):M110.007385. Epub 2011 Feb 1. 10.1074/mcp.M110.007385 PubMed 21285413